Microscope
Kara Gene Tashkhis is a provider of bright field -invert-fluorescent microscopes from reputable companies such as Olympus-Zeiss-Nikon in pathobiology , genetics laboratories and infertility centers.
Bright field microscope:
A bright-field microscope, also known as a compound light microscope is among the simplest of optical microscopes. Optical microscopes employ visible light and a series of lenses to magnify the specimen and view it in detail.
A bright-field microscope uses light rays to create a dark image against a bright background and it is this contrast that makes this microscope simple and effective for use.
A bright-field microscope’s ability to create a high-resolution image from a well-illuminated light source that is focused on the specimen make it a simple yet effective microscope.
Inverted microscope:
An inverted microscope is a microscope with its light source and condensor on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up.
The invert technique is a very popular method for examining living cells. in This Technique samples are being evaluated from the bottom of the cell culture flask.
Fluorescent microscope:
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study the properties of organic or inorganic substances. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation while phosphorescence is a specific type of photoluminescence related to fluorescence. Unlike fluorescence, a phosphorescent material does not immediately re-emit the radiation it absorbs. The fluorescence microscope was devised in the early part of the twentieth century by August Köhler, Carl Reichert, and Heinrich Lehmann, among others.
Most cellular components are colorless and cannot be clearly distinguished under a microscope. The basic premise of fluorescence microscopy is to stain the components with dyes.
Fluorescent dyes, also known as fluorophores or fluorochromes, are molecules that absorb excitation light at a given wavelength (generally UV), and after a short delay emit light at a longer wavelength. The delay between absorption and emission is negligible, generally on the order of nanoseconds.
The emission light can then be filtered from the excitation light to reveal the location of the fluorophores.
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